Solubilization of the protective antigens ofBordetella pertussis

Antigens of Bordetella pertussis V. Separation of Agglutinogen 1 and Mouse-Protective Antigen.

Agglutinogen 1 of Bordetella pertussis strain 353/Z (serotype 1) was separated from protective antigen and histamine-sensitizing factor by starch-block electrophoresis. Most of the agglutinogen 1 migrated towards the cathode in starch-block electrophoresis, although some remained near the origin. Fractions containing most of the agglutinogen 1 were free of detectable mouse-protecting or histami...

Experimental study of interference between Pertussis antigens and Salk Poliomyelitis vaccine

Solubilization and Characterization of a Protective Antigen of Erysipelothrix rhusiopathiae.

A particulate fraction of Erysipelothrix rhusiopathiae cultures has been subjected to butanol extraction and treatment with surface-active agents in an attempt to solubilize a protective antigen. The particulate fraction was partitioned into the butanol layer but was not solubilized. Only sodium dodecyl sulfate solubilized the particles. The soluble protective activity was not sedimented by cen...

Protective antigens in experimental schistosomiasis.

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Identification of the protective and toxic antigens of Corynebacterium pseudotuberculosis.

Cameron (1964) showed that the immunizing property of Corynebacterium pseudotuberculosis cells was closely associated with the toxicity of cell endoplasm and the ability of formalin-killed cells to produce sterile abscesses. He also found that the presence of toxic factors in the cells depended on the culture medium to a large extent. Furthermore Cameron & Swart (1965) developed a fluid medium ...